Temperature Sensitivity Complementation, Cell Cycle Specific, H142

Five separate temperature-sensitive (ts) cell cycle specific mutations in cultured cell lines are known to be complemented by human genes, each situated on a different chromosome (Ming, 1984). The ts mutant Syrian hamster cell line AF8 (116950) shows arrest in G1, 8.6 hours before the S phase; the complementing human gene is on chromosome 3. The H142 ts mutant is complemented by a gene on chromosome 9 (Baserga et al., 1982). Arrest at nonpermissive temperatures occurs at the early S phase.